Vitamin Analysis of water-soluble vitamins by HPLC-water-soluble vitamins in cell culture media and aqueous stock premix.
Cell culture media are comprised of complex mixtures of nutrients to support cell growth. Ongoing quality assurance of stock premixes is a cost effective way of insuring that acceptable levels of vitamins are going into media production batches. Samples taken from reactors can be used to determine whether a vitamin is a limiting nutrient for cell production.
Method summary for analysis of vitamins
Method development is underway at AminoAcids.com to add additional assays for other vitamins in cell culture media.
The complexity of the sample matrix and the low concentration of vitamins can affect the LLQ.
Samples need to be protected from light and refrigerated or frozen.